Application

Anti-HAP1, clone 1B6, Cat. No. MABN2456, is a highly specific mouse monoclonal antibody that targets Huntingtin-associated protein 1 and has been tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Immunocytochemistry Analysis: A representative lot detected HAP1 in Immunocytochemistry applications (Fujinaga, R., et. al. (2011). Exp. Cell Res. 317(12):1689-700).

Immunoprecipitation Analysis: A representative lot immunoprecipitated HAP1 in Immunoprecipitation applications (Fujinaga, R., et. al. (2011). Exp. Cell Res. 317(12):1689-700).

Western Blotting Analysis: A representative lot detected HAP1 in Western Blotting applications (Chan, E.Y., et. al (2002). Hum Mol Genet. 11(8):945-59; Tang, T.S., et. al. (2003). Neuron. 39(2):227-39; Torre, E.R., et. al. (2003). J Neurosci Methods. 125(1-2):27-32).

Research CategoryNeuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Huntingtin-associated protein 1 (UniProt: P54256; also known as HAP1) is encoded by the Hap1 gene (Gene ID: 29430) in rat. HAP1 is a huntingtin-associated protein that is enriched in the brain. It specifically associates with HTT/huntingtin and this binding is enhanced by an expanded polyglutamine repeats within HTT that affects HAP1 interaction properties. Both HTT and HAP1 are reported to be involved in intracellular trafficking and HAP1 is proposed to link HTT to motor proteins and/or transport cargos. HAP1 localizes to large nonmembrane-bound cytoplasmic bodies (stigmoid bodies) in various types of neurons. The formation of stigmoid bodies is regulated by the ratio of isoform A to isoform B of HAP1. HAP1 plays a role in vesicular transport within neurons that involves microtubule-associated transporters and is shown to be attenuated by association with mutant HTT. In rodents, two HAP1 isoforms (HAP1A and HAP1B) have been described that differ in their carboxy terminal. However, humans contain only the HAP1A isoform. In murine models, targeted disruption of the HAP1 gene results in significantly depressed feeding behavior and premature postnatal death. Pups with Hap1 -/- show decreased feeding, malnutrition, dehydration, and premature death. (Ref.: Tang, TS et al., (2003). Neuron 39 (2); 227-239; Chan, EY., et al. (2002). Hum. Mol. Gen. 11(8); 945-959).

Immunogen

Epitope: cytoplasmic domain

GST-tagged recombinant fragment corresponding to 137 amino acids from the internal region of rat Huntingtin-associated protein 1 (HAP1).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Format: Purified

Quality

Evaluated by Western Blotting in brain tissue lysate from P1 Balb/c mouse.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected HAP1 in brain tissue lysate from day one post-natal (P1) Balb/c mouse.

Specificity

Clone 1B6 detects both HAP1A and HAP1B proteins in mouse and rat brain. It targets an epitope from the internal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~90 kDa observed; 70.21 kDa calculated. Uncharacterized bands may be observed in some lysate(s).